RUNX1 and acute myeloid leukemia: Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and targeted deep sequencing of YCU-AML1 cells confirmed high EVI1 expression and mutational profile consistent with recently published mutational spectrum of myeloid malignancies with inv(3)/t(3;3).14 Therefore, YCU-AML1 cells and our PDX model can be used as functional tools for seeking molecular basis of high-risk human AML-MRC with t(3;3)(q21;q26.2) and monosomy 7.