To confirm that this was due to apoptosis, cleaved caspase-3, a key mediator of programmed cell death was stained for in HCT116, OE33, and KYSE70 cancer cells by immunocytofluorescence in cancer cells treated with varying concentrations of the plant extract (LC50 × 2, LC50, and LC50/2) (Figures 1C–E). This evidence concerns the gene CASP3 and cancer.