Moreover, the tube formation results indicated that HG dramatically impaired the angiogenesis ability of HUVECs, while the reduction of p65 expression induced by Bay11-7082 under HG situation recovered the angiogenesis ability of HUVECs (Figure 5D), suggesting that the antiangiogenic effect of HG (or GDM) is mediated at least in part by activation of p65. This evidence concerns the gene RELA and gestational diabetes.