In HCC cells, SIRT1 had a predominant nuclear localization where its expression promotes tumorigenesis, while, cytoplasmatic SIRT1 may have tumor-suppressive roles 94, 95.Although SIRT1 appears to be a promising target for preventing ALD, there are few studies that have reported the roles of SIRT1 in ethanol-fed HCC due to lack of appropriate models 96. Here, SIRT1 is linked to hepatocellular carcinoma.