In the process of mitochondrial glutaminolysis, glutamine is initiated by amino acid transporter ASCT2 (alanine-serine-cysteine transporter 2; SLC1A5), and then enter the TCA cycle through being converted to glutamate by glutaminase GLS1/2 and subsequently to α-ketoglutarate (α-KG), which can provide energy and biological macromolecular materials for tumor cell survival 11. Here, GLS is linked to neoplasm.