A fraction of type II strains vacuoles spontaneously differentiate to bradyzoites in normal culture conditions, as evidenced by SAG2Y expression on the parasite surface and labeling of the tissue cyst walls with Dolichos biflorus agglutinin (DBA), which recognizes O-linked N-acetylgalactosamine moieties on wall proteins (e.g., CST1) (12). Here, RPS19 is linked to cyst.