Thus, CCL21 was determined after NF-κB was antagonized and results revealed that the CCL21 secretion was significantly inhibited and the metastasis of A549 cell in the co-culture system was also decreased, which suggested that TNF-α-mediated CCL21 secretion in HLEC associated with NF-κB signaling and the lymphocytic metastasis of NSCLC cells. Here, TNF is linked to non-small cell lung carcinoma.