Because miR‐34a‐5p was shown to be up‐regulated in patients with migraine, we down‐regulated miR‐34a‐5p by using its inhibitor, and then we detected the expression level of SIRT1, IL‐1β, miR‐34a‐5p, NF‐κB p65 and COX2 by quantitative real‐time PCR. The gene discussed is NFKB1; the disease is migraine disorder.