In order to identify the potential targets of BAP1, lysates from prostate cancer cells P69, M12, DU145, PC3, and BPH1 (a benign prostatic hyperplasia epithelial cell line) as well as HeLa cells were used for western blotting analysis, showing that the protein levels of PTEN, which is a crucial regulator for the PI3K‐Akt pathway, were positively correlated with BAP1, except that in PC3 cells which is lack of PTEN expression [42] (Fig. 3B). This evidence concerns the gene ICA1 and benign prostatic hyperplasia.