The univariate analysis suggested that tumor histological type, differentiation degree, Nevin stage, liver metastasis, vascular invasion, lymph node metastasis, resection method, NOX4 expression in tumor cells/GCAFs were significant prognostic indicators for the OS of GBC patients (all P < 0.05). Here, NOX4 is linked to metastatic malignant neoplasm in the lymph nodes.