As shown in Table 1-2, chi-square analysis and spearman rank correlation analysis indicated that the expression of NOX4 in tumor/stromal cells was not related to age, gender, tumor location, tumor size, histological type, lymph node metastasis and resection method (all P > 0.05), but significantly associated with tumor differentiation, liver metastasis, vascular invasion, Nevin staging and VM (all P < 0.05) in GBC patients. This evidence concerns the gene NOX4 and benign neoplasm.