To get a first impression about the differential expression and activation of, e.g., angiogenesis-related proteins and signaling pathways in mock-transfected (A375-pIRES) versus EphB4-overexpressing A375 melanoma cells (A375-EphB4), we performed both the Cancer BioMarker Antibody Array (SCB200) and the Cancer Signaling Phospho Antibody Array (PCS248) together with the Antibody Microarray kit (KAS02) (Figure 5a,b). The gene discussed is EPHB4; the disease is melanoma.