In order to better substantiate our results with this subsequent investigation, also with regard to the pathomechanisms, we analyzed both expression and activation of, e.g., angiogenesis-related proteins and signaling pathways in mock-transfected (A375-pIRES) versus EphB4-overexpressing A375 melanoma cells (A375-EphB4). This evidence concerns the gene EPHB4 and melanoma.