Consistent with the findings from the array, pyrosequencing showed that methylation of AVP cg16536918 and LGR6 cg03566881 were significantly associated with GDM exposure (AVP cg16536918, r2 = 0.334, p = 0.012; LGR6 cg03566881, r2 = 0.263, p = 0.049) and 1-h PG (AVP cg16536918, r2 = 0.343, p = 0.0034; LGR6 cg03566881, r2 = 0.272, p = 0.019). The gene discussed is LGR6; the disease is gestational diabetes.