To explore which isoform of LHFPL3-AS1 was required for maintaining the stemness of melanoma stem cells, the expression of LHFPL3-AS1 isoforms was respectively overexpressed in the LHFPL3-AS1-knocked-down melanoma stem cells by transfecting the vector expressing LHFPL3-AS1-long or LHFPL3-AS1-short (Fig. 2G). The gene discussed is PTGDR; the disease is melanoma.