Given that Foxp3 expression is regulated by epigenetic changes, as methylation of CpG sites in the foxp3 locus, we isolated Tregs from wild-type mice affected with experimental periodontitis (10 days after periodontitis induction) and baseline controls to compare the methylation levels of 4 CpG sites at the TSDR region (Fig. 4D). Here, FOXP3 is linked to periodontitis.