We detected a sharp reduction in the Foxp3 MFI on CD4+CD25+Foxp3+ cells after 5- and 10-days post-periodontitis induction at all cervical lymph-nodes (≈ 28% reduction) and spleens (≈ 17% reduction) (Fig. 3B,C), although the effect was more significant in cervical lymph nodes. This evidence concerns the gene CD4 and periodontitis.