APP and amyotrophic lateral sclerosis: To validate the contribution of APP in MN degeneration in our in vitro models of ALS, we assessed its expression in astrocytes and found that the APP mRNA levels were 128 ± 12% (mean ± SEM of n = 3 independent experiments; 95% confident interval [CI]: 105–152%) higher in mutSOD1 than in NTg astrocytes.