To define the signaling pathways that differ between CARs with CD28.ζ (CD28-CAR), 41BB.ζ (41BB-CAR), and MyD88.CD40.ζ (MC-CAR), we generated CARs specific for EphA2, a tumor-associated antigen broadly expressed in brain and solid tumors (18, 19). The gene discussed is EPHA2; the disease is neoplasm.