INS and Hypoinsulinemia: The short-term group received a single high dose of STZ (shown to exert direct toxicity on insulin-producing cells, leading to necrosis within 2–3 days, ref. 42) that led to hypoinsulinemia (–58%), overt basal (nonfasted) hyperglycemia (+4-fold), and a mild loss of body weight (–18%) (Supplemental Figure 8A).