TP53 and benign prostatic hyperplasia: Furthermore, western blotting analysis showed that in rMIF treatment, the expression of COX-2 was significantly upregulated and the expression of P53 was downregulated compared with control, while in rMIF+celecoxib treatment, the expression of COX-2 and the expression of P53 were partially reversed in BPH-1 (Fig. 7A,B) and PWR-1E cells (Fig. 7C,D).