Further analysis revealed an increase in cleaved caspase-3, caspase-8, caspase-9, and poly-ADP ribose polymerase-1 (PARP-1), along with the reduced expression of Bcl-2 and increased expression of Bax indicating the combination of TSA and BEZ235 causes an anti-breast cancer effect through both mitochondrial pathway and the death receptor pathway (Figure 3). This evidence concerns the gene PARP1 and breast cancer.