To determine whether TNF-α, IL-1α, and C1q transformation of A1 astrocytes plays a role in RGC death in the microbead-induced eIOP model of glaucoma, WT, Il1a−/−; Tnf−/− double knockout (DKO) mice, C1qa−/− single knockout mice, and Il1a−/−; Tnf−/−; C1qa−/− (TKO) mice were injected with magnetic microbeads in one eye and BSS in the fellow eye. Here, IL1A is linked to glaucoma.