Pyruvate kinase M2 (Pkm2) is a limiting glycolytic enzyme capable of catalysing the last step in glycolysis.14 Interestingly, Pkm2 has been found to increase neurogenesis and promote functional recovery in rats after ischaemic stroke.15 In addition, Pkm2 can interact with Mfn2 to accelerate mitochondrial fusion, thus, to modulate cancer cell growth.16 Herein, the current study was conducted to explore whether miR‐214 could regulate mitochondrial fusion in brain injury via regulating Mfn2‐Pkm2 interaction in rats. This evidence concerns the gene MFN2 and ischemic stroke.