Aiming to better characterise the function of the secreted acid trehalase, in the present report we have deleted the CgATH1 gene from the C. glabrata genome and analysed the phenotypic consequences in the mutant strain, including the growth pattern when exposed to glucose or trehalose rich mediums, the trehalase activity, and its virulence in a murine model of infection, either in normal or immunocompromised mice. The gene discussed is TREH; the disease is infection.