Additionally, Nurfaradilla et al. [42] described that in the male Sprague-Dawley 2K1C hypertension model (4 weeks), the plasma renin level, serum angiotensin-converting enzyme (ACE) activity, and plasma angiotensin II level were significantly elevated in the 2R1C group compared to the SHAM group indicating that the increased plasma renin level was likely by increasing renal production in response to loss of perfusion. Here, ACE is linked to hypertensive disorder.