TP53 and cancer: R273H mutation is one of the hotspot mutations in TP53 in human cancers.8,11,12 We first conducted a large-scale co-immunoprecipitation with a FLAG antibody following overexpression of TP53R273H-FLAG in U251MG cells (TP53R273H-harbouring human glioblastoma cell line34) (Supplementary Fig. S1).