We found that the tumor-infiltrating T cells highly expressed regulatory and exhausted markers, such as TIGIT (LogFoldChange = 0.258224344, adjusted p value = 2.75e−143), LAYN (LogFoldChange = 0.071034001, adjusted p value = 1.90e−12), FOXP3 (LogFoldChange = 0.082017301, adjusted p value = 7.92e−13) and CTLA4 (LogFoldChange = 0.101004531, adjusted p value = 4.63e−13), while the T cells in normal tissues highly expressed effector and naïve T-cell markers (Fig. 3b, c, Supplementary Fig. S4). This evidence concerns the gene CTLA4 and neoplasm.