RPS6KB1 and breast carcinoma: As shown in Supplementary Fig. 1, the exposure of breast cancer cells to rapamycin not only suppressed the activity of the mTORC1 axis molecules such as decreased p-p70S6K (T389) and p-S6 (S240/244) but also caused a marked increase in p-Akt both at S473 and T308 in a time- and dose-dependent manner.