Staining of cells from the developed cultures showed the presence of markers for premeiotic (VASA), meiotic (BOULE), and meiotic/postmeiotic (ACROSIN) cells (in control culture without IL-34; CT and IL-34-treated cultures; IL-34), whereas cells before culture (BC) were stained only for VASA cells, but not for BOULE or ACROSIN (Figure 4B). This evidence concerns the gene IL34 and breast cancer.