ACTA1 and intrahepatic cholangiocarcinoma: Several studies have shown that MSCs are affected by the surrounding microenvironment, becoming themselves protagonists of the disease development [25]; for this reason, MSCs were further characterized by IIF/ICC and qRT-PCR through the expression of α-SMA, collagen type 1 (COL1A1), fibronectin, and TGF-β1.