Methyl donor supplementation containing folic acid (a synthetic version of folate), betaine, choline and vitamin B12 also reverted hepatic fat accumulation in high-fat and high-sucrose (HFS) diet-fed rats by affecting the DNA methylation patterns in the promoter regions of sterol regulatory element-binding protein 2 (Srebf2), 1-acylglycerol-3-phosphate O-acyltransferase 3 (Agpat3) and estrogen receptor 1 (Esr1) genes, which are involved in obesity development and lipid metabolism, and modifying their mRNA expression [80]. The gene discussed is ESR1; the disease is obesity disorder.