They found that both DNA methylation and mRNA expression of tumor-related genes, such as tripartite motif-containing 4 (TRIM4), protein regulator of cytokinesis 1 (PRC1) and tubulin alpha 1b (TUBA1B), were altered in NASH-T samples, but not in viral-T, compared to NLT and confirmed that these DNA methylation changes were observed even in NASH-N samples at the precancerous NASH stage. The gene discussed is TUBA1B; the disease is neoplasm.