In ballooned hepatocytes, CK8/18 highlights Mallory-Denk bodies (MDB) that are aggregates of ubiquitinated keratins within the proteasome [37]; therefore, double immunostaining for CK8/18 and ubiquitin has been used for the detection of hepatocyte ballooning in NASH [38]. This evidence concerns the gene KRT8 and metabolic dysfunction-associated steatohepatitis.