PPARG and metabolic dysfunction-associated steatotic liver disease: Hardy et al. [136] demonstrated that two specific CpG dinucleotides at the promoter region of PPARγ, which is a negative regulator of HSC activation and liver fibrogenesis [28], were hypermethylated in plasma cfDNA isolated from patients with NAFLD with severe fibrosis compared to patients with NAFLD with mild fibrosis.