The EMT marker SNAIL increased over 3-fold at the mRNA level, and interleukin 8 (IL-8) and matrix metalloproteinase 9 (MMP9) increased approximately 2-fold and 3-fold, respectively, at the protein level in HS5 cells after CML EV treatment. Here, MMP9 is linked to chronic myelogenous leukemia, BCR-ABL1 positive.