The results show that the phosphorylation of JNK signaling was higher in CTCs after the treatment by 20 dyne/cm2 than 0 dyne/cm2 shear stress in multiple breast cancer cells, including SKBR3, MDA-MB-468, and MCF-7 (Figure 3a and Figure S3a,c). This evidence concerns the gene MAPK8 and breast carcinoma.