SAMHD1 and infection: We showed this by (1) directly visualizing continued nuclear vDNA synthesis in MDMs (see also [9]), which progressed more efficiently in the absence of SAMHD1 (Figure 5), and (2) corroborating this result in TZM-bl and CHME3 cells by correlating the kinetics of single HIV-1 nuclear import and productive integration with the completion of vDNA synthesis and infection assessed by a time of drug addition assay (Figure 1C and Figure S2B).