WB samples collected from HDs and MM patients were coincubated with K562, 721.221, or P815-ULBP1+CD48 target cells for 24 h in the presence of 100 U/mL IL-2 as the activation with IL-2 amplified the NK cell response without hampering the performance of the assay. The gene discussed is ULBP1; the disease is Miyoshi myopathy.