SQSTM1 and Cachexia: Compared to unloaded (7-day I and 15-day I) animals, in mice with the two conditions (LC-cachexia and unloading), beclin-1, p62, LC3-II/I, and cleaved caspase-3 protein levels did not significantly differ, while LC3-II/I levels (only in LC 30-days + 15-day I) significantly increased (+80% change, Figure 5A–E).