First, univariate regression analysis demonstrated that 22 genes had a relationship with poor prognosis, especially SLC10A2, PLCG2, and PTH1R. Previous reports have mentioned that secondary bile acids increase the risk of COAD, and SLC10A2 plays a crucial role in intestinal bile acid reabsorption. The gene discussed is SLC10A2; the disease is colon adenocarcinoma.