We found that a transfer of exclusively CD4+CD44−Foxp3GFP− Tn cells from EpTCRmini mice to lymphopenic EpTCRαk/o recipients resulted in lethal cachexia in <8 weeks (Fig. 3a), demonstrating that a single AbEp complex activated autologous CD4+ Tn cells. The gene discussed is CD4; the disease is Cachexia.