To study the effects of APOE genotype on AD-related pathways in a physiologically relevant environment, we generated 3-D cerebral organoid models using human iPSC lines from cognitively unimpaired individuals carrying APOE ε3/ε3 (Con-E3; N = 5), cognitively unimpaired individuals carrying APOE ε4/ε4 (Con-E4; N = 5), AD patients carrying APOE ε3/ε3 (AD-E3; N = 5) and AD patients carrying APOE ε4/ε4 (AD-E4; N = 5), collected from multiple sources (Supplementary Table 1). The gene discussed is APOE; the disease is Alzheimer disease.