APOE and Alzheimer disease: We further quantified G3BP, endosome marker protein early endosome antigen 1 (EEA1), and lysosome marker protein lysosomal-associated membrane protein 1 (LAMP1) by western blotting, and found the interactive effect between APOE4 and AD status; G3BP significantly increased in organoids from AD patients carrying APOE4 (Fig. 6f, g).