Furthermore, induction of DNA double-stranded breaks with the NCS treatment (140 ng/mL for 1 h at 37 °C) results in a ~2 fold increase in γH2AX and 53BP1 foci in shRAD51-expressing cells compared to the shSCM-GBM counterparts (Figure 3C,D). This evidence concerns the gene TP53BP1 and glioblastoma.