After filtering out synonymous mutations and removing the unmatched records, we had ∼3.9 million somatic mutation records from the five cancer-related datasets and further obtained 679 374 somatic mutations associated with human PKs assigning on 180 856 unique AA positions (abbreviated as AAps) (Figure 2C, COSMIC: 392 345 AAps, ICGC: 162 557 AAps, TCGA: 66 528 AAps, CCLE: 34 096 AAps, GDSC: 23 848 AAps, Table 1). Here, ARAF is linked to cancer.