Therefore, we subsequently investigated the frequency of different T cell subgroups after melatonin treatment and found that the frequency of CD4+ T cells expressing either IFNγ or TNFα was lower in melatonin-treated PBMCs than in vehicle-treated PBMCs from either MG patients or HCs (Figure 2C, 2D). The gene discussed is CD4; the disease is myasthenia gravis.