Engineered iPSCs-derived liver organoids helped in illustrating the various functions that different mutations of JAG1 gene can have in the development of bile ducts and genesis the Alagille syndrome: the C829X mutation of JAG1 can causes significant alterations, while the G274D mutation does not affect organoid properties (Guan et al. 2017). The gene discussed is JAG1; the disease is Alagille syndrome.