Results of RT-PCR in 56 osteosarcoma tissues revealed that expression of PLOD1 was higher in patients with lung metastasis presented at diagnosis than those without metastasis (Figure 1(d), P < 0.01), while PLOD2 and PLOD3 expression presented no significance between two groups (Figures 1(e) and 1(f), all P > 0.05). This evidence concerns the gene PLOD2 and osteosarcoma.