TXNRD1 and central nervous system cancer: To investigate whether inhibition of TrxR1 by UMAs induces oxidative stress in glioma cell lines with different antioxidative capacities (Stankovic et al., 2015; Stojkovic et al., 2015), we assessed the levels of RONS (peroxynitrite anion and hydrogen peroxide) with fluorescent dye DHR by flow cytometry (Supplementary Figure 2).