EGFR and cancer: Target prediction analysis showed that miR-141-3p potentially targets Mapk8 (Figure 7C), which is involved in the MAPK signaling pathway, ErbB signaling pathway, and pathways in cancer When we analyzed Mapk8 expression in F2-ovaries using RT-qPCR, we observed that miR-141-3p expression increased in F2 milk-siblings, whereas Mapk8 expression decreased compared to the control group (Figure 7D).