To mimic clinical symptoms of C-IRIS, Rag1–/– mice were reconstituted with CD4+ T cells (106 cells/mouse) by adoptive transfer 3 weeks after infection with Cryptococcus neoformans serotype A H99 (CnH99, 100 yeasts/mouse) (Supplementary Figure 1A), unless otherwise noted. This evidence concerns the gene RAG1 and infection.