After 6 h, uninfected cells remain unperturbed (Figure 8(b-x)(c-x)), those infected with HB101 show very mild or no disruption of occludin or ZO-1 (Figure 8(b-xi)(c-xi)), and as expected, tEPEC-E2348/69 infection induces more severe effects (Figure 8(b-xii)(c-xii)). This evidence concerns the gene TJP1 and infection.