Conversely, across two C9-ALS iPSC MNs, 14% and 61% of MATR3 puncta, respectively, colocalized with G4C2 RNA, while a significant portion of MATR3 puncta occurred independent of G4C2 foci (Additional file 2: Figure S2B). Here, MATR3 is linked to amyotrophic lateral sclerosis.