HMGB1 is defined as one of the significantly differential genes (Fig. 1a), and further Western blot analyses validated its higher expression in the bone marrow mononuclear cells (MNCs) of AML patients compared with the normal controls (Fig. 1b), which is in accordance with the findings previously reported [18] and implies important role of HMGB1 in the leukemogenesis of AML. This evidence concerns the gene HMGB1 and acute myeloid leukemia.