SP14-C8 CAR-transduced Jurkat cells were also potently activated in the presence of an antigen-positive MCF-7 breast cancer cell line and CHO–EpCAM cells, although the SP12-E10 CAR was not active in this assay, possibly due to the activation kinetics of the cells, the constraints imposed by a CAR-T configuration compared with that of a soluble scFv, or the lower affinity of this antibody. Here, EPCAM is linked to breast cancer.