To further qualitatively visualize the increased CXCL1 expression in mouse tumor tissue, we performed IHC and show that in both mutants R248W and R248Q, the R72 SNP maintains relatively higher levels of CXCL1 expression compared to their P72 counterparts (Figure 4C, Supplementary Figure S5A,B). Here, CXCL1 is linked to neoplasm.